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primary antibody pgam1  (Abbexa Ltd)


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  • 90

    Structured Review

    Abbexa Ltd primary antibody pgam1
    Primary Antibody Pgam1, supplied by Abbexa Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+pgam1/primary+antibody+pgam1/pm30698749-88-87-93
    Average 90 stars, based on 1 article reviews
    primary antibody pgam1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.
    Article Snippet: Samples of 20 μg of protein homogenizing were retrieved from the testes of the control groups and the exposed to EDCs groups (n = 3) and separated by electrophoresis on a 12% polyacrylamide gel (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) under denaturing and reducing conditions and then transferred to a nitrocellulose membrane (Thermo Scientific, USA) at 350 mA for 2 h. Next, membranes were blocked with a solution of 3% (w/v) bovine serum albumin 0.1% (v/v) Tween in Trisbuffered saline, pH 7.4 and incubated overnight with the respective primary antibodies for PGAM1 (0.4 ng/μl) (Abbexa, UK) and ß-ACTIN (0.3 μg/μl) (Sigma-Aldrich, USA) as a loading control.

    Electrophoresis:

    Article Title: Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.
    Article Snippet: Samples of 20 μg of protein homogenizing were retrieved from the testes of the control groups and the exposed to EDCs groups (n = 3) and separated by electrophoresis on a 12% polyacrylamide gel (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) under denaturing and reducing conditions and then transferred to a nitrocellulose membrane (Thermo Scientific, USA) at 350 mA for 2 h. Next, membranes were blocked with a solution of 3% (w/v) bovine serum albumin 0.1% (v/v) Tween in Trisbuffered saline, pH 7.4 and incubated overnight with the respective primary antibodies for PGAM1 (0.4 ng/μl) (Abbexa, UK) and ß-ACTIN (0.3 μg/μl) (Sigma-Aldrich, USA) as a loading control.

    Nucleic Acid Electrophoresis:

    Article Title: Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.
    Article Snippet: Samples of 20 μg of protein homogenizing were retrieved from the testes of the control groups and the exposed to EDCs groups (n = 3) and separated by electrophoresis on a 12% polyacrylamide gel (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) under denaturing and reducing conditions and then transferred to a nitrocellulose membrane (Thermo Scientific, USA) at 350 mA for 2 h. Next, membranes were blocked with a solution of 3% (w/v) bovine serum albumin 0.1% (v/v) Tween in Trisbuffered saline, pH 7.4 and incubated overnight with the respective primary antibodies for PGAM1 (0.4 ng/μl) (Abbexa, UK) and ß-ACTIN (0.3 μg/μl) (Sigma-Aldrich, USA) as a loading control.

    Membrane:

    Article Title: Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.
    Article Snippet: Samples of 20 μg of protein homogenizing were retrieved from the testes of the control groups and the exposed to EDCs groups (n = 3) and separated by electrophoresis on a 12% polyacrylamide gel (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) under denaturing and reducing conditions and then transferred to a nitrocellulose membrane (Thermo Scientific, USA) at 350 mA for 2 h. Next, membranes were blocked with a solution of 3% (w/v) bovine serum albumin 0.1% (v/v) Tween in Trisbuffered saline, pH 7.4 and incubated overnight with the respective primary antibodies for PGAM1 (0.4 ng/μl) (Abbexa, UK) and ß-ACTIN (0.3 μg/μl) (Sigma-Aldrich, USA) as a loading control.

    Saline:

    Article Title: Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.
    Article Snippet: Samples of 20 μg of protein homogenizing were retrieved from the testes of the control groups and the exposed to EDCs groups (n = 3) and separated by electrophoresis on a 12% polyacrylamide gel (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) under denaturing and reducing conditions and then transferred to a nitrocellulose membrane (Thermo Scientific, USA) at 350 mA for 2 h. Next, membranes were blocked with a solution of 3% (w/v) bovine serum albumin 0.1% (v/v) Tween in Trisbuffered saline, pH 7.4 and incubated overnight with the respective primary antibodies for PGAM1 (0.4 ng/μl) (Abbexa, UK) and ß-ACTIN (0.3 μg/μl) (Sigma-Aldrich, USA) as a loading control.

    Incubation:

    Article Title: Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.
    Article Snippet: Samples of 20 μg of protein homogenizing were retrieved from the testes of the control groups and the exposed to EDCs groups (n = 3) and separated by electrophoresis on a 12% polyacrylamide gel (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) under denaturing and reducing conditions and then transferred to a nitrocellulose membrane (Thermo Scientific, USA) at 350 mA for 2 h. Next, membranes were blocked with a solution of 3% (w/v) bovine serum albumin 0.1% (v/v) Tween in Trisbuffered saline, pH 7.4 and incubated overnight with the respective primary antibodies for PGAM1 (0.4 ng/μl) (Abbexa, UK) and ß-ACTIN (0.3 μg/μl) (Sigma-Aldrich, USA) as a loading control.



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